… of vector copies in DNase I treated vs untreated nuclear … between species by treating
transduced cells with DNase I prior … from degradation in the +DNase I condition in both species. …

… regulating this heterogeneity is the chromatin state in each cell. … Using budding yeast, we
have developed a system to track … The primers used for real-time PCR are listed in Table 2. …

… track shows a composite DO mESC ATAC-seq track (median normalized sequencing depth
across all DO mESC samples). The lower tracks show published DNaseI … 7 Real-Time PCR …

… By monitoring chromatin states and mRNA stability upon … Reverse transcription and
real-time quantitative PCR were … Genomic DNA was removed by in solution DNaseI treatment (…

… Programming viral chromatin states by capsid design may … between species by treating
transduced cells with DNAse I prior to … in the same manner as the nuclei were used as control. …

… with quantitative real-time polymerase chain reaction (PCR), is … for monitoring the differentiation
state of organoids in real time. … , exhibiting a closed chromatin state. These domains are …

… Z mimicked the Per null chromatin state and disrupted cellular … 100 nM dexamethasone, and
real-time bioluminescence was … (Iprasense) by continuous monitoring of cell cultures inside …

… Real-time PCR analysis confirmed that 6-MP treatment or … To address this, we performed
stable isotope tracing … extracted using RNeasy Mini kit (Qiagen) including a DNase I …

… Monitoring proliferation over six days revealed decreased … using RNeasy Kit (Qiagen),
followed by DNase digestion … performed on a StepOnePlus Real-Time PCR machine. For each …

… independently to maintain repressive chromatin states. … on a StepOnePlus™ Real-Time
PCR system. To examine … We introduced a novel temporal dimension by monitoring changes in …